The Ultimate Guide To high performance liquid chromatography
The Ultimate Guide To high performance liquid chromatography
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The mobile period carries the sample parts with the column, in which they communicate with the stationary section to varying degrees. This interaction decides just how long Every element spends while in the column, leading to their separation.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
Switching the cell period’s composition because the separation progresses is just one Alternative to this problem. For any reversed-stage separation we use an Preliminary cell phase that may be more polar. Since the separation progresses, we change the composition of mobile stage to make sure that it becomes much less polar (see Figure twelve.5.6
Gradient optimization: In gradient elution, the cell period composition variations over time. An improperly intended gradient may result in bad resolution. Critique your gradient profile and adjust the gradient slope or solvent ratios to attain improved separation concerning analytes of fascination.
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高速液体クロマトグラフィーにおいては各物質は比較的鋭いピークとして検出され、分離（他の物質のピークと明確に分けられる）および検出（鋭いピークにより高い感度が得られる）の能力が従来の液体クロマトグラフィーより良くなる。
The order of elution of compounds within the column is governed with the depth of connection with the stationary section. The get more info eluent Using the separated chemicals flows previous the detector.
we acquired how to adjust the cell stage’s polarity by Mixing collectively two solvents. A polarity index, however, is just a information, and binary mobile period mixtures with identical polarity indices might not solve Similarly a set of solutes. Table 12.5.2
The cellular phase’s stream charge is determined by the merged speeds of the two pumps. By changing the relative speeds of the two pumps, unique binary cell phases could be organized.
The pressurized liquid is often a combination of solvents including drinking water, acetonitrile and/or methanol and is often called the cellular phase.
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Resolution: Exact injection minimizes band broadening, which can lead to overlapping peaks and hinder separation.